Replacement Beta-Cells From An Unexpected Source

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Dr.Lancman in Lab

Replacement Beta-Cells From An Unexpected Source

Dr.Lancman in Lab Project Researcher: Joseph Lancman, Ph.D. – Sanford Burnham Prebys Medical Discovery Institute

Project Description

A cure for diabetes will require replacing the insulin-producing beta-cells that have been lost in this disease. Replacing these lost cells in patients with diabetes can be achieved through transplantation of donor beta-cells, allowing recipients to live their life free from daily insulin injections. While promising, the major challenge now lies in finding a source of replacement beta-cells to treat the millions with diabetes.

Our lab has recently discovered a way to reprogram cells to become a completely unrelated cell type without having to remove them from the body. This discovery may allow us to generate new, replacement beta-cells directly in people with diabetes and bypass the safety issues associated with other approaches currently being investigated.

The prevailing approach that many researchers are taking to help produce more donor beta-cells is to generate them from cultured stem cells. But many safety concerns still need to be addressed, including the tumor-forming potential of these cultured cells and their rejection by the recipient’s immune system. Recognizing these concerns, researchers are exploring the use of encapsulation devices to prevent transplanted cells from leaking into the body. However, using devices to encapsulate cells raises new issues including device efficacy, durability and implantation site scarring.

Given the concerns associated with transplantation of cells cultured from stem cells, our lab sought a completely different approach to generate replacement beta-cells. Using our knowledge of how cell identity, particularly pancreas cells, is genetically programmed and our new theory of cell identity regulation, we recently devised a method to reprogram completely unrelated cells into cells that can normally go on to form beta-cells. With this breakthrough, we believe we have made a substantial advance towards converting nearly any cell type of our choice into replacement beta-cells, without having to remove them from a patient’s body.

Efforts to reprogram the identity of cells without removing them from the body (in vivo) have been restricted to converting cells that are already closely related to beta-cells. However, these cells come from tissue like the pancreas and liver that may already be stressed in diabetics, making their use potentially harmful to the patient. Instead of targeting these cells, our discovery suggests that it may be possible to directly generate replacement beta-cells from more abundant and nonessential cells, such as fat, skin, and muscle cells, and potentially from immune privileged tissues within a patient’s body. This approach would bypass the need for potentially harmful cultured cells, invasive surgeries, bulky encapsulation devices, and immunosuppressants.

To make in vivo cell lineage reprogramming practical and safe, we aim to expand the types of cells that can be converted into beta-cells. Our vision is to reprogram a small subset of cells directly into beta-cells from select, nonvital tissues throughout the body without disrupting the normal functions of that tissue. We have now overcome a major limitation of in vivo cell lineage reprogramming by converting completely unrelated cells into cells that can go on to form beta- cells. It is now imperative that we continue to push and further guide our in vivo induced naïve cells into functional beta-cells. With your support, we will be able to accelerate our proof of concept studies to determine the feasibility of using in vivo cell lineage reprogramming to generate a vast new supply of replacement beta-cells.

Project Updates

Update on 3-06-18

Over the past few months, we have been resolved to optimize our isolation of reprogrammed muscle cells so we can really start to understand what kind of molecular changes are occurring as the muscle cells change identity in vivo. With this in-depth understanding, we think we will be better able to guide the reprogrammed muscle cells further along towards an early pancreatic cell identity and ultimately a beta-like cell identity.

And after several months, it is with great pleasure that I am excited to let you know that we have finally (finally!) overcome our technical problems! We were finally able to successfully isolate many reprogrammed muscle cells following our experiment! With this advance, we can now combine isolated reprogrammed muscle cells from 2 or 3 experiments and perform the in-depth molecular analysis we had originally proposed to do.

Because of the problems we were having isolating large numbers of reprogrammed muscle cells, I mentioned in my last update that we began pursuing another type of analysis that did not require us to isolate so many reprogrammed muscle cells. This alternative approach allows us to isolate individual reprogrammed cells, and perform analysis on each separately, rather than analysis on a large pool of cells, like our other technique. After having discussed our experimental details with experts in this single cell analysis technique, we have concluded that this alternative strategy to analyze individual reprogrammed muscle cells will also be feasible!

One month ago, we were stalled as we attempted to isolate enough reprogrammed muscle cells for in-depth analysis. But over the past month, we have found  TWO viable ways to perform in-depth molecular analysis of in vivo reprogrammed muscle cells! This is really exciting and shows you how fast our fortunes can change in science when we continue to push and work hard. This is a major step for us as we continue to pursue our goal of in vivo reprogramming of cell identity towards early endoderm identity and eventually beta cell identity!

Thank you for hanging in there with us. It’s been a bumpy ride. But we will continue to make progress and push forward!!!

Until next month……..

Update on 11-30-17

With the holiday season upon us, it’s a good time to step back and reflect on our past year and be thankful for all the positive events we have been associated with. This also applies to science, especially when things aren’t going as well as we’d like. If you recall, our goal for the previous month was to try and continue to optimize the number of reprogrammed muscle cells we collect. This is because we had issues collecting enough reprogrammed cells to perform in-depth analysis of the changes that are occurring within these reprogrammed muscle cells.

Unfortunately, we have not yet been able to improve our yields of reprogrammed cells. But we are continuing to work hard to improve our outcomes. Importantly, these challenges we have encountered have provided an opportunity to try some new ideas on how to increase the number of reprogrammed cells we are getting. One of which I’d like to share with you. As you know, we use two factors to change the identity of muscle cells into early endoderm-like cells, the cells that can normally go on to form beta cells in the body. These factors we use are a special type of protein called a transcription factor. Transcription factors are primarily found inside a cell’s nucleus where they can turn genes on and off.

One of our ideas was to try and make the transcription factors we are using better at turning on the genes needed for early endoderm identity. What is really amazing is that many other researchers have modified transcription factors to make them more active or better at what they normally do. In a sense, they are like super versions of themselves! After looking at how others have made transcription factors better, more potent, we have decided on a few changes that may enhance each of the transcription factors we use. To make the changes, we needed to either add new parts or change specific sequences in the mRNA of the transcription factors. We have completed the changes to one of our factors and are now working on making changes to the other. Once we make the changes to both, we can test them and determine whether they enhance reprograming in vivo.

We are excited about this approach and believe it could be an exciting new way to enhance the reprogramming that we are trying to accomplish in vivo. It should also help us collect more reprogrammed cells in order to carry out the analysis we would like to perform. We also believe that, ultimately, by having more reprogrammed cells to start with, we will have more opportunities, and therefore a better chance, to push their identity closer to beta-cells.

So enjoy the holiday season and keep in mind that we are working hard to fix our technical problems. The sooner we can clear this hurdle, the sooner we can continue to try and push reprogrammed cells towards a beta-cell identity in vivo!

Update on 10-31-17

Sometimes in research (often!), things don’t go quite as planned. The past month has been a challenge in regards to our research efforts and as a result, our progress has been slowed.

Our goal for the past month was to try and continue to optimize the number of reprogrammed muscle cells we can collect in order to do in-depth analysis on the changes that are occurring within these cells. Recall that we are trying to look at all the changes that occur in mRNA expression levels in reprogrammed muscle cells compared to normal, control muscle cells that haven’t been reprogrammed. By analyzing the changes, we can get a better idea of what else we need to do to push the identity of reprogrammed muscle cells closer to a beta cell identity.

Because each cell we collect only gives us a tiny amount of mRNA, we need to collect many, many cells in order to gather enough mRNA to do in-depth analysis. Unfortunately, we still haven’t been able to increase the number of reprogrammed muscle cells that we can collect at a single time. So, we are generating new ideas and are still confident will be able to generate and collect the larger numbers of reprogrammed muscle cells we need to carry out our analysis.

We believe that most of our problems are stemming from how we are delivering the reprogramming factors. The most likely problem is that we are not getting enough into the cells. What’s interesting is that we can still learn a lot about the biology of reprogramming from the problems we encounter.

So what can we learn from our current problems? It seems that the amount of reprogramming factors we are using and putting into the cells may be a critical parameter for success. Too little, and reprogramming doesn’t occur at high enough incidence. On the other hand, putting in too much of the reprogramming factors might also reduce our reprogramming efficiency. We can say this because those performing reprogramming of cells in vitro have already documented this phenomenon. Therefore, we can tentatively infer that, like those working to reprogram cells in vitro, we need to be careful to add just the right amount of reprogramming factors to achieve optimal levels of reprogramming.

More importantly, because we are experiencing some of the same problems people working on in vitro cell reprogramming have experienced, it means we can learn a lot from them and apply it to our problem(s)! So don’t get discouraged. These delays always occur in research. With hard work and creative problem solving, we’ll get through it. Stay tuned as we continue to solve our technical problems and keep pushing forward….

Update on 9-30-17

Hello! It’s time again to give you all a quick update on our progress over the past month. As I mentioned in my last update, we were working hard to iron out some of the technical problems we were having as we attempted to isolate reprogrammed muscle cells that were changing their identity.

The reason we need to isolate these reprogrammed muscle cells is so we can carefully analyze and characterize how their identity change is occurring. Armed with that information, we can then determine what other changes we need to make in order to continue to push reprogrammed muscle cells into beta cells.

Below is a cartoon to remind you what we are trying to do:

A key part of our experiments that I think is important for you to understand is our need to also isolate and analyze control muscle cells. Control muscle cells are important because they do NOT undergo reprogramming and therefore tell us what mRNA normal muscle cells usually express. Knowing this, we can then compare the types of mRNA that normal muscle cells express to the mRNA that reprogrammed muscle cells express, allowing us to detect differences in mRNA expression between the two.

Over the past month we have repeated our experiments several times and tweaked several parameters in order to increase the number of reprogrammed and control muscle cells. This allowed us to isolate more reprogrammed or control muscle cells per experiment. Even though we were able to collect more cells, we still haven’t been able to collect enough cells to do really in depth analysis. However, we were able to isolate enough reprogrammed and control muscle cells to do important preliminary analysis.

In our preliminary analysis of the molecular changes in reprogrammed muscle cells, we could detect expression of mRNAs that are only expressed in early naive gut like cells! Importantly, we never detected these mRNA in control muscle cells! This means that our analysis will work and that reprogrammed muscle cells are in fact changing their identity and beginning to express mRNA that is important for formation of early naive gut like cells.

So, for the next month, we will continue to tweak our experimental conditions in order to get more reprogrammed muscle cells and control muscle cells. This will allow us to do more in depth and complete analysis of the molecular changes that are occurring during muscle cell reprogramming.

More cells! More analysis! Until next month……

Update on 8-31-17

The ultimate goal is to determine whether we can transform completely unrelated cells in the body into functional beta-cells by successfully reprogramming muscle or skin cells into a naive gut like cell that can go on to form tissue like the pancreas (where beta cells normally develop). We need to carefully analyze and characterize how this change is occurring so we can determine what other changes need to be made in order to continue pushing these reprogrammed muscle or skin cells into beta cells. Going forward, we will initially focus our efforts on reprogramming and analyzing muscle cells in order to thoroughly understand the molecular changes that occur while reprogrammed muscle cells change identity in vivo, this requires isolation of the reprogrammed muscle cells by separating them from muscle cells that have not been reprogrammed (see Diagram below).

Over the past month, we have made great progress in working out the proper conditions to efficiently deliver our conversion factors and the fluorescent tag to be sure that a muscle cell always receives both the factors and the fluorescent tag and still remains healthy. We also had to be sure that the fluorescent tag itself did not influence our reprogramming efforts (it does not). Although not fully optimized, we have had enough success to begin trial runs of cell sorting using FACS.

Over the next month, we will continue to optimize the delivery of the fluorescent tag and the cell sorting. During this time, the goal is to isolate enough reprogrammed muscle cells in order to begin in depth analysis of the molecular changes that are occurring. Armed with this information, we can begin making plans for how to try to keep pushing these cells closer to a functional beta cell!”

Update on 7-1-17

We have cleared a major hurdle in our efforts to convert completely unrelated cells in the body into beta-cell pre-cursors. We have discovered that delivering two factors into muscle or skin cells can convert them into a naive gut cell type that can go on to form tissue like the pancreas, where beta cells normally develop. In order to now try and continue to push these reprogrammed muscle or skin cells into beta cells, we need to thoroughly understand the molecular process that occurs while they change identity.

Over the next 3-4 months, I will be analyzing converted cells in order to determine the critical molecular changes that occur as they change their identity. These experiments will yield data necessary to inform our next strategy to drive these cells closer to becoming functional beta cells.

By successfully moving this project forward, we are exploring a fundamentally novel therapeutic approach for beta-cell replacement. It is also creating an opportunity to help those who deal with the complications of diabetes every day of their life. The experiments I am currently focused on should lead to new and exciting insights into changing cell identity in vivo and bring us a few steps closer to our goal of generating beta-cells in the body. And it is because of your generous support and the support of the DRC that it is possible.

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