Imbalance of Bacteriome Profiles Within the Finnish Diabetes Prediction and Prevention Study

Diabetes Supplies - Bottles

Original article published by Wiley Online Library on August 22, 2016. Click here to read the original article.

Abstract

Background

We set out to explore associations between the stool bacteriome profiles and early-onset islet autoimmunity, taking into account the interactions with the virus component of the microbiome.

Methods

Serial stool samples were longitudinally collected from 18 infants and toddlers with early-onset islet autoimmunity (median age 17.4 months) followed by type 1 diabetes, and 18 tightly matched controls from the Finnish Diabetes Prediction and Prevention (DIPP) cohort. Three stool samples were analyzed, taken 3, 6, and 9 months before the first detection of serum autoantibodies in the case child. The risk of islet autoimmunity was evaluated in relation to the composition of the bacteriome 16S rDNA profiles assessed by mass sequencing, and to the composition of DNA and RNA viromes.

Results

Four operational taxonomic units were significantly less abundant in children who later on developed islet autoimmunity as compared to controls—most markedly the species of Bacteroides vulgatus and Bifidobacterium bifidum. The alpha or beta diversity, or the taxonomic levels of bacterial phyla, classes or genera, showed no differences between cases and controls. A correlation analysis suggested a possible relation between CrAssphage signals and quantities of Bacteroides dorei. No apparent associations were seen between development of islet autoimmunity and sequences of yet unknown origin.

Conclusions

The results confirm previous findings that an imbalance within the prevalent Bacteroidesgenus is associated with islet autoimmunity. The detected quantitative relation of the novel “orphan” bacteriophage CrAssphage with a prevalent species of the Bacteroides genus may exemplify possible modifiers of the bacteriome.

[su_button url=”http://onlinelibrary.wiley.com/doi/10.1111/pedi.12468/full” target=”blank” style=”flat” background=”#64b243″ size=”6″ center=”yes” radius=”5″ icon=”icon: angle-right”]Continue Reading[/su_button]

Related News

CONNECTING FOR A CURE: June 2022 Newsletter

Learn More +

Diabetes Research Connection Awards $400,000 to Eight Promising T1D Studies

Learn More +

Meet a DRC Volunteer: Kaya Keutler

Learn More +